• Document: Agilent Seahorse XF Live-Cell Metabolism Solutions for STEM CELL RESEARCH
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Agilent Seahorse XF Live-Cell Metabolism Solutions for STEM CELL RESEARCH MONITOR CELL FATE TRANSITIONS Improve Differentiation and Reprogramming Outcomes Adult Fibroblast Cells Stem Cells Hematopoietic Cells Adipocytes Reprogramming Differentiation (iPS Cells) (iPS/ES Cells) Cardiomyocytes Hepatocytes Neural Cells Cellular age and origin, in addition to donor variability, protocol differences, growth rates and media choices all contribute to inconsistent reprogramming and/or differentiation efficiencies. Metabolic energy utilization, characterized before and after cell fate changes occur, identifies the metabolic phenotype and enables researchers to predict and confirm cell function, revealing actionable reprogramming and differentiation potential. Cellular metabolic phenotyping measures the cell’s energy requirement and pathway preference for readying the transition between undifferentiated and differentiated states. Metabolic switching occurs rapidly as cells transition from quiescent to pluripotent and/or from pluripotent to differentiated. Seahorse XF Technology: • Live cell • Real-time • Label-free • Dynamic injection ports • Measures oxygen consumption and glycolytic rates simultaneously Somatic oxidative bioenergetics transitions into pluripotency- dependent glycolysis to facilitate nuclear reprogramming. 2 Folmes, C. D., et al. Cell Metab. 2011. 14: 264-71. THE POWER OF CELL METABOLIC PHENOTYPING Identify Pluripotency and Differentiation Transitions Seahorse XF technology enables reliable Cardiomyocyte Neuron Adipocyte Fibroblast measurements that predict, monitor, and track Reprogramming cell fate transitions. Discover how these metabolic measurements can be used as indicators to minimize inefficiencies and improve differentiation Differentiation and reprogramming approaches. Routine assays Stem Cell iPS Cell make identifying cell phenotype and cell transitions easy. What’s more, the metabolic phenotyping analysis that Seahorse XF delivers provides the Glycolysis Oxidative Metabolism Glycolysis tools and knowledge to customize your approach, Metabolic plasticity in stem cell homeostasis and differentiation. and push the conventional boundaries of stem cell Folmes, C. D., et al. Cell Stem Cell. 2012. 11: 596-606. research through the development of new assays. Seahorse XF Technology: • Measures distinct metabolic signatures • Characterizes cellular phenotypes at each stage • Enables routine and reliable stem cell phenotyping • Facilitates the discovery of new standards and benchmarks Seahorse XF Technology simultaneously mea

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